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 CV MIROSLAV RADMAN

Extension CV of Miroslav Radman

 

 

 

 

 

 

 CV MIROSLAV RADMAN

Extension CV of Miroslav Radman

 

Phenotypic and Genetic Consequences of Protein Damage

Phenotypic and Genetic Consequences of Protein Damage

PLoS Genetics Krisko-Radman. Protein damage reduces the efficacy and precision of vital cellular processes resulting in high mutation rates and functional degeneracy akin to cellular aging...

Extreme anti-oxidant protection against ionizing radiation in bdelloid rotifers

Extreme anti-oxidant protection against ionizing radiation in bdelloid rotifers

We conclude that the great radioresistance of bdelloid rotifers is a consequence of an unusually effective system of anti-oxidant protection of cellular constituents, including those required for DSB repair, allowing bdelloids to recover and continue reproducing after doses of IR causing hundreds of DSBs per nucleus. Bdelloid rotifers therefore offer an advantageous system for investigation of enhanced anti-oxidant protection and its consequences in animal systems.

Stoichiometry of MutS and MutL at unrepaired mismatches in vivo suggests a mechanism of repair. Elez M, Radman M, Matic I. Source

Stoichiometry of MutS and MutL at unrepaired mismatches in vivo suggests a mechanism of repair. Elez M, Radman M, Matic I. Source

Our results corroborate the hypothesis postulating that MutL accumulation assures the coordination of the MMR activities between the mismatch and the strand discrimination site.

Three Major Research Projects

Three Major Research Projects

This three tier project offers, at itsR & D stage,original science-based solutions to three major problems of humanity: healthy longevity, local food supplyand local energy supply. Mission and purpose of the first two projects is to profoundly change the public health by combining an original molecular diagnostics with a new treatment employing natural compounds, ofnutriceutical kind, that is both preventive and therapeutic.

Biology of robustness

Biology of robustness

Life’s robustness depends on the resilience of fertile organisms (the soma) that assure the long-term evolutionary success of the germ-line, i.e., species. In the framework of this project we explore the chemistry of two types of biological clocks: the species-specific somatic clock (robustness of the proteome and life span) and the universal germ-line clock (mutations and evolution). As model organisms, we explore the bacterium D. radiodurans and aquatic animals bdelloid rotifers as well as complex animals like tardigrades, all equally resistant to long-term desiccation and extreme doses of ionizing radiation.

RecA protein assures fidelity of DNA repair and genome stability in Deinococcus radiodurans.

RecA protein assures fidelity of DNA repair and genome stability in Deinococcus radiodurans.

Deinococcus radiodurans is one of the most radiation-resistant organisms known. It can repair hundreds of radiation-induced double-strand DNA breaks without loss of viability. Genome reassembly in heavily irradiated D. radiodurans is considered to be an error-free process since no genome rearrangements were detected after post-irradiation repair. Here, we describe for the first time conditions that frequently cause erroneous chromosomal assemblies.

Protein damage and death by radiation in Escherichia coli and Deinococcus radiodurans.

Protein damage and death by radiation in Escherichia coli and Deinococcus radiodurans.

Deinococcus radiodurans is among a small number of bacterial species that are extremely resistant to ionizing radiation, UV light, toxic chemicals, and desiccation. We measured proteome oxidation (i.e., protein carbonylation, PC) in D. radiodurans as well as in standard and evolved resistant strains of Escherichia coli exposed to ionizing radiation or UVC light and found a consistent correlation with cell killing. The unique quantitative relationship between incurred PC and cell death holds over the entire range of killing for all tested bacteria and for both lethal agents, meaning that both bacterial species are equally sensitive to PC.

Unstructured hydrophilic sequences in prokaryotic proteomes correlate with dehydration tolerance and host association.

Unstructured hydrophilic sequences in prokaryotic proteomes correlate with dehydration tolerance and host association.

Here, we explore possible hallmarks of prokaryotic desiccation tolerance in their proteomes. The content of unstructured, low complexity (LC) regions was analyzed in a total of 460 bacterial and archaeal proteomes. It appears that species endowed with proteomes abundant in unstructured hydrophilic LC regions are desiccation-tolerant or sporulating bacteria, halophilic archaea and bacteria, or host-associated species.

Biological responses to DNA damage

Biological responses to DNA damage

DNA Repair Systems and Bacterial Evolution - Book Biological responses to DNA damage; page 11 - 19.

in the BOOK: SOS Hypothesis and the Emergence of Integrative Biology

in the BOOK: SOS Hypothesis and the Emergence of Integrative Biology

A major discovery can often be recognized by the need to coin a new word (e.g., atom, radioactivity, antibiotic, apoptosis, etc). If the discovered entity or phenomenon does not have its cognate word in our vocabulary, it is surely a breakthrough. Coining the word SOS response (SOS system, SOS replication, SOS repair) to describe coordinated multiple inducible cellular responses to DNA damage resulting in induced mutagenesis and cell survival, was such a privilege (Radman 1974). This is a personal historical account of the intellectual circumstances that led to the birth of SOS hypothesis

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Miroslav Radman - Molecular Biology and Genetics Scientist